![]() ![]() ![]() Thus, there is a need to develop lower cost methodologies that do not require specialized equipment. In many labor and delivery settings outside of research hospitals, maintaining cryopreservation capabilities for deliveries that can happen at any hour of any day is difficult and can be expensive. At present, placental metabolic profiling studies typically rely on flash freezing of specimens 5, 6. Despite the increased interest in measuring placental metabolism, few studies have incorporated these measures, which may be due, at least in part, to challenges involved in collecting specimens suitable for metabolic profiling. Placental metabolic processes in particular may offer insight into potential mechanisms linking suboptimal intrauterine exposures to childhood health outcomes and could also suggest therapeutic and nutritional interventions to mitigate the adverse effects of fetal programming on offspring. Interest in incorporating placental functional measures and related information into human studies on the developmental origins of health and disease has increased in recent years and has expanded from measurements such as placental weight, thickness, and cotyledon number to epigenetics, magnetic resonance imaging, and metabolomics 1, 2, 3, 4. These results show that placental tissue samples collected in 80% methanol at room temperature can yield similar metabolic data to flash frozen specimens. Principle components analysis demonstrated separation of the metabolite features in the methanol extract, but similarity between methanol-fixed tissue and flash frozen tissue. In positive ion mode, when compared to flash frozen tissue, both the methanol extract and methanol-fixed tissue (6 h) had a higher number of metabolites detected (146 additional metabolites, p FDR = 0.020 149 additional metabolites, p FDR = 0.017 respectively), but these associations were not found in negative ion mode (all p FDR ≥ 0.05). Methanol-fixed tissue samples and methanol extracts had a similar number of metabolites (p = 0.45, p = 0.21 in positive vs. Data were analyzed using Gaussian generalized estimating equations, two sample t-tests with false discovery rate (FDR) corrections, and principal components analysis. Untargeted metabolic profiling was performed on both the methanol-fixed tissue and the methanol extract. Specimens were excised from the maternal side of the placenta and immediately flash frozen or fixed and stored for 1, 6, 12, 24, or 48 h in 80% methanol. In such instance, we will not be liable for any changes in quality of your order.Methods for collection of placental tissue at room temperature for metabolic profiling are described. If no one is home to receive it despite our delivery crew's best efforts, the order will be left at your doorstep. What if I'm unable to receive my order at time of delivery?Ī notification will be sent prior to delivery. Unfortunately, we are unable to deliver to Sentosa or Tuas for the time being.Ĩ. Please note that delivery and self collection will not be available on Sundays.Īn additional surcharge of $4 will be included for deliveries on (a) islandwide delivery, except Sentosa and Tuas, at a flat rate of $10īoth delivery and self collections will be completed between 12 - 8pm (Mon-Fri) and 12 - 4pm (Sat). diabetic) should exercise caution and avoid consumption of raw food.įor orders placed by 2359hrs, we offer the following options for next day But as a general precaution, vulnerable groups of people, especially young children, pregnant women, elderly persons or people with chronic illness (e.g. Once thawed, they should be consumed as soon as possible for freshness. The frozen oysters can be stored in the freezer for 2 - 3 months. Raw, grilled, steamed, baked, fried in any method you fancy.įor a taste of the sweet and fruity flavour profile, we do highly recommend enjoying them raw in the og style - a couple drops of lemon and a dash of tabasco. Drain the water and use a spoon or knife to reveal the succulent flesh within. oysters should be fully submerged in water) for 20 - 25 minutes. Rinse the oysters under running water to remove debris, then thaw them in a water bath (i.e. Our oysters are unshucked, but they are easy to open with just a spoon. For this reason, the oysters remain fresh and can be consumed raw. Yes, they are flash frozen at time of harvest to maintain the cells of the oysters. Are the oysters frozen? If so, can I still enjoy them raw? ![]()
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